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Human VEGFC protein, His tag (active)

Human VEGFC protein, His tag (active). GTX00104-pro
Human VEGFC protein, His tag (active). GTX00104-pro
Human VEGFC protein, His tag (active). GTX00104-pro

Cat. No. GTX00104-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($299)
See all VEGFC protein products

APPLICATION

Application Note

Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. And VEGFC significantly promoted cell proliferation of ECV304 cells. The ED₅₀ for this effect is typically 0.304-1.339μg/ml.

Observed MW

45 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu47~Trp413 (NP_005420.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vascular endothelial growth factor C , Flt4-L , LMPH1D , LMPHM4 , VRP

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Human VEGFC protein, His tag (active). GTX00104-pro

GTX00104-pro Functional Assay Image

The dose effect curve of GTX00104-pro Human VEGFC protein (active) on ECV-304 cells. After incubation of Human VEGFC protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human VEGFC protein, His tag (active). GTX00104-pro

GTX00104-pro Image

WB analysis of GTX00104-pro Human VEGFC protein (active).

Human VEGFC protein, His tag (active). GTX00104-pro

GTX00104-pro Image

SDS-PAGE analysis of GTX00104-pro Human VEGFC protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299