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Human alpha 1 Antitrypsin protein, His tag (active)

Human alpha 1 Antitrypsin protein, His tag (active). GTX01220-pro

Cat No. GTX01220-pro

Application

Functional Assay

Species

Human
Package
20 μg ($329),
10 μg ($249)

APPLICATION

Application Note

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC₅₀ value is < 3.0 nM, as measured in 100μl reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

Observed MW

55-60 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Lys418 of Human alpha 1 Antitrypsin protein (NP_000286.3)

Expression System

HEK293 cells

Purity

> 97% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

A1A,A1AT,AAT,PI,PI1,PRO2275,alpha1AT,nNIF

Background

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human alpha 1 Antitrypsin protein, His tag (active). GTX01220-pro

GTX01220-pro Image

SDS-PAGE of 5 μg GTX01220-pro Human alpha 1 Antitrypsin protein, His tag (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 329
$ 249