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Human alpha Synuclein protein (Pre-Formed Fibrils)

Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro
Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro
Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro
Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro
Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro

Cat No. GTX17667-pro

Application Functional Assay
Species Human
Package
200 μg ($599),
100 μg ($399)
PRODUCT

Product Description

Human Recombinant Alpha Synuclein Preformed Fibrils (Type 2)
APPLICATION

Application Note

Does not induce Lewy body inclusion formation in Sprague-Dawley rat primary hippocampal neurons. Thioflavin T emission curve shows only a small increase in fluorescence (indicative of alpha synuclein aggregation) when Type 2 alpha synuclein PFFs (GTX17667-pro) are combined with alpha synuclein monomers (GTX17668-pro or GTX17666-pro). Certain biological activities in other neuronal cells cannot be ruled out. Researchers should test compatibility prior to use.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length without tagged; MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Expression System

E. coli

Purification

Purified by ion-exchange chromatography

Purity

92 % by SDS-PAGE

Endotoxin

< 1EU/μg (by LAL assay)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro

GTX17667-pro Functional Assay Image

Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Preformed Fibrils (GTX17669-pro) at 4 µg/ml (D-F), but not when treated with control Alpha Synuclein Preformed Fibrils (GTX17667-pro) at 4 µg/ml (A-C).
Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.

Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro

GTX17667-pro Image

SDS-PAGE of ~14 kDa Human alpha Synuclein protein (fibrils) (GTX17667-pro).
Lane 1: Molecular Weight Ladder (MW). Lane 2: Human alpha Synuclein protein (fibrils) (GTX17667-pro).

Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro

GTX17667-pro Image

TEM of active human alpha synuclein preformed fibrils (GTX17669-pro) (top) and control human alpha synuclein preformed fibrils (GTX17667-pro) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the control fibrils.

Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro

GTX17667-pro Image

TEM of active human alpha synuclein preformed fibrils (GTX17669-pro) (top) and control human alpha synuclein preformed fibrils (GTX17667-pro) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the control fibrils.

Human alpha Synuclein protein (Pre-Formed Fibrils). GTX17667-pro

GTX17667-pro Image

Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 µM of alpha synuclein monomer (GTX17668-pro), as compared to when 10 nM of control alpha synuclein preformed fibrils (GTX17667-pro) is combined with 100 µM of alpha synuclein monomer (GTX17668-pro) or 100 µM of alpha Synuclein monomer (GTX17666-pro). Thioflavin T ex = 450 nm, em = 485 nm.

REFERENCE
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REVIEW
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Package List Price ($)
$ 599
$ 399