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Human alpha Synuclein protein (active, monomer)

Human alpha Synuclein protein (active, monomer). GTX17668-pro
Human alpha Synuclein protein (active, monomer). GTX17668-pro

Cat. No. GTX17668-pro

Application

 Functional Assay

Species

 Human
Package
200 μg ($689),
100 μg ($439)
See all alpha Synuclein protein products

APPLICATION

Application Note

100 μM alpha synuclein protein monomer (GTX17668-pro) seeded with 10 nM alpha synuclein protein pre-formed fibrils (GTX17669-pro) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37ºC with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length without tagged; MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Expression System

E. coli

Purification

Purified by ion-exchange chromatography

Purity

> 95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synuclein alpha , synuclein alpha , NACP , PARK1 , PARK4 , PD1

Database

  • Gene ID: 6622 SNCA

Research Area

DATA IMAGES

Human alpha Synuclein protein (active, monomer). GTX17668-pro

GTX17668-pro Image

SDS-PAGE of ~14 kDa Human alpha Synuclein protein (active, monomer) (GTX17668-pro).
Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (5 μg). Lane 3: BSA (2.5 μg). Lane 4: Human alpha Synuclein protein (active, monomer) (5 μg) (GTX17668-pro). Lane 5: Human alpha Synuclein protein (active, monomer) (2.5 μg) (GTX17668-pro).

Human alpha Synuclein protein (active, monomer). GTX17668-pro

GTX17668-pro Image

Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro), as compared to when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha Synuclein monomer (GTX17666-pro). Thioflavin T ex = 450 nm, em = 485 nm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 689
$ 439