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Human alpha Synuclein protein (monomer)

Human alpha Synuclein protein (monomer). GTX17666-pro
Human alpha Synuclein protein (monomer). GTX17666-pro

Cat No. GTX17666-pro

Application

Functional Assay

Species

Human
Package
200 μg ($609),
100 μg ($409)

APPLICATION

Application Note

Thioflavin T curve shows less β-sheet aggregation when Type 2 monomers (GTX17666-pro) are seeded with PFFs compared to Type 1 monomers (GTX17668-pro) seeded with PFFs.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length without tagged; MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Expression System

E. coli

Purification

Purified by ion-exchange chromatography

Purity

92 % by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , synuclein alpha , NACP , PARK1 , PARK4 , PD1

Database

  • Gene ID: 6622 SNCA

Research Area

DATA IMAGES

Human alpha Synuclein protein (monomer). GTX17666-pro

GTX17666-pro Image

Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro), as compared to when 10 nM of control alpha synuclein preformed fibrils (GTX17667-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro) or 100 μM of alpha Synuclein monomer (GTX17666-pro). Thioflavin T ex = 450 nm, em = 485 nm.

Human alpha Synuclein protein (monomer). GTX17666-pro

GTX17666-pro Image

SDS-PAGE of ~14 kDa Human alpha Synuclein protein (active, monomer) (GTX17666-pro).
Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (2.5 μg). Lane 3: BSA (5 μg). Lane 4: Alpha Synuclein Protein Monomer (2.5 μg) (GTX17666-pro). Lane 5: Alpha Synuclein Protein Monomer (5 μg) (GTX17666-pro).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 609
$ 409