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Human alpha Taxilin protein (active)

Human alpha Taxilin protein (active). GTX00218-pro
Human alpha Taxilin protein (active). GTX00218-pro
Human alpha Taxilin protein (active). GTX00218-pro

Cat No. GTX00218-pro

Application

Functional Assay

Species

Human
Package
10 μg ($299)

APPLICATION

Application Note

Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TXLNa and recombinant human PDIA3. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDIA3 was in a dose dependent manner.

Observed MW

33 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys328~Glu531 (NP_787048.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

taxilin alpha , IL14 , TXLN

Database

DATA IMAGES

Human alpha Taxilin protein (active). GTX00218-pro

GTX00218-pro Functional Assay Image

Functional ELISA analysis of GTX00218-pro Human alpha Taxilin protein (active) which can bind immobilized PDIA3 protein.

Human alpha Taxilin protein (active). GTX00218-pro

GTX00218-pro Image

SDS-PAGE analysis of GTX00218-pro Human alpha Taxilin protein (active).

Human alpha Taxilin protein (active). GTX00218-pro

GTX00218-pro Image

WB analysis of GTX00218-pro Human alpha Taxilin protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 299