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Human alpha Taxilin protein (active)

Human alpha Taxilin protein (active). GTX00218-pro
Human alpha Taxilin protein (active). GTX00218-pro
Human alpha Taxilin protein (active). GTX00218-pro

Cat No. GTX00218-pro

Application Functional Assay
Species Human
Package
10 μg ($299)
APPLICATION

Application Note

Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TXLNa and recombinant human PDIA3. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDIA3 was in a dose dependent manner.

Observed MW

33 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys328~Glu531 (NP_787048.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

taxilin alpha,IL14,TXLN

Database

DATA IMAGES
Human alpha Taxilin protein (active). GTX00218-pro

GTX00218-pro Functional Assay Image

Functional ELISA analysis of GTX00218-pro Human alpha Taxilin protein (active) which can bind immobilized PDIA3 protein.

Human alpha Taxilin protein (active). GTX00218-pro

GTX00218-pro Image

SDS-PAGE analysis of GTX00218-pro Human alpha Taxilin protein (active).

Human alpha Taxilin protein (active). GTX00218-pro

GTX00218-pro Image

WB analysis of GTX00218-pro Human alpha Taxilin protein (active).

REFERENCE
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Package List Price ($)
$ 299