Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MX1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was in a dose dependent manner.
Reconstitute with 20mM Tris and 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
N-terminal His-Tag; Val170~Val419 (NP_001280141.1)
< 1 EU/μg
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
tubulin beta class I , CDCBM6 , CSCSC1 , M40 , OK/SW-cl.56 , TUBB1 , TUBB5
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]