4 μM p23 Protein, when added to 2 μM AHA1 Protein (Aha1)-activated HSP90 (2 μM; His-tagged HSP90 beta) in 33 mM HEPES (pH 7.2) with 30 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 1.5 mM ATP in a 100 μl reaction at 37 ºC, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
20 mM HEPES buffer (pH 7.2), 80 mM NaCl, 10% glycerol.
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Full-length protein; NP_006592.3 with N-terminal SH residue
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
prostaglandin E synthase 3 , P23 , TEBP , cPGES
This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes. [provided by RefSeq, Feb 2016]