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Human v-Myc protein (active)

Human v-Myc protein (active). GTX00126-pro
Human v-Myc protein (active). GTX00126-pro
Human v-Myc protein (active). GTX00126-pro
Human v-Myc protein (active). GTX00126-pro

Cat No. GTX00126-pro

Application

Functional Assay

Species

Human
Package
10 μg ($309)

APPLICATION

Application Note

MYC (Myc proto-oncogene protein) is a nuclear phosphoprotein that binds specific sequence of DNA. MYC functions as a transcription factor and regulates transcription of target genes. It has been proven that c-Myc protein is intracellularly associated with TBP (TATA-binding protein) of the TFIID transcription initiation complex; besides, TRRAP (Transformation/transcription domain-associated protein) is thought to be an essential cofactor for the MYC. Thus a binding ELISA assay was conducted to detect the interaction of MYC with TBP and TRRAP. Briefly, recombinant human MYC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TBP-coated and TRRAP microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MYC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MYC with TBP and TRRAP was shown in images, and this effect was in a dose dependent manner.

Observed MW

65 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser184~Ala454 (NP_002458.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Human v-Myc protein (active). GTX00126-pro

GTX00126-pro Functional Assay Image

Functional ELISA analysis of GTX00126-pro Human v-Myc protein (active) which can bind immobilized TRRAP protein.

Human v-Myc protein (active). GTX00126-pro

GTX00126-pro Functional Assay Image

Functional ELISA analysis of GTX00126-pro Human v-Myc protein (active) which can bind immobilized TBP protein.

Human v-Myc protein (active). GTX00126-pro

GTX00126-pro Image

SDS-PAGE analysis of GTX00126-pro Human v-Myc protein (active).

Human v-Myc protein (active). GTX00126-pro

GTX00126-pro Image

WB analysis of GTX00126-pro Human v-Myc protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 309