WB analysis of endogenous HTT lysates with or without different caspase activity (Lanes 1-5) and overexpressed recombinant HTT fragment lysates (Lanes 6-9) using a N-terminal pan-HTT antibody (upper panel) or GTX54521 Huntingtin (neoepitope 552) antibody at 1:500 (lower panel).
Loading : 20 μg

ICC/IF analysis of 293T cells transfected with an Htt23Q (left panel) and Htt148Q (right panel) stop constructs ending in amino acid 552 using GTX54521 Huntingtin (neoepitope 552) antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Sandwich ELISA was performed with a monoclonal anti- Humtingtin antibody and GTX54521 Huntingtin (neoepitope 552) antibody to determine the antigen concentration of the Htt cleavage products. The curve represents a dose response for neo552 in 293T cells overexpressing the Htt construct.

IHC-Fr analysis of mouse cortex tissue (from transgenic HD mice of the YAC128 line) using GTX54521 Huntingtin (neoepitope 552) antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:50