WB analysis of endogenous HTT lysates with or without different caspase activity (Lanes 1-5) and overexpressed recombinant HTT fragment lysates (Lanes 6-9) using GTX54522 Huntingtin (neoepitope 586) antibody (Bottom panel) and pan-HTT antibody (Top panel).
Dilution : 1:500
Loading :20 μg

IHC-Fr analysis of mouse striatum (top panels) and cortex (bottom panels) tissue from either transgenic HD mice of the YAC128 line (left panels) or their WT littermates (right panels) using GTX54522 Huntingtin (neoepitope 586) antibody.
Green : Primary antibody
Dilution : 1:50

ICC/IF analysis of 293T cells transfected with Htt23Q and Htt148Q stop constructs ending in amino acid 586 using GTX54522 Huntingtin (neoepitope 586) antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Sandwich ELISA was performed with a Huntingtin monoclonal antibody and GTX54522 Huntingtin (neoepitope 586) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo586 in 293T cells overexpressing the Htt construct.