GeneTex
United States (US)

Huntingtin (neoepitope 586) antibody

GTX54522_983_IHC-Fr_19011519_940.jpg
GTX54522_2015_ICC-IF_19011519_412.jpg
GTX54522_81_ELISA_19011519_424.jpg
GTX54522_2084_WB_19011519_597.jpg

Cat No. GTX54522

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:50-1:200
IHC-Fr 1:50 - 1:200
ELISA 1:20 - 1:100
Not tested in other applications.

Calculated MW

348 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes the 586 cleaved fragment without detecting the full-length form.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.60mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide conjugated to KLH via cysteine corresponding to CPSDSSEIVLD (577-586) of Human HTT.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HD,IT15,LOMARS

Cellular Localization

Cytoplasm,Nucleus

Background

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES
GTX54522_983_IHC-Fr_19011519_940.jpg

GTX54522 IHC-Fr Image

IHC-Fr analysis of mouse striatum (top panels) and cortex (bottom panels) tissue from either transgenic HD mice of the YAC128 line (left panels) or their WT littermates (right panels) using GTX54522 Huntingtin (neoepitope 586) antibody.
Green : Primary antibody
Dilution : 1:50

GTX54522_2015_ICC-IF_19011519_412.jpg

GTX54522 ICC/IF Image

ICC/IF analysis of 293T cells transfected with Htt23Q and Htt148Q stop constructs ending in amino acid 586 using GTX54522 Huntingtin (neoepitope 586) antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

GTX54522_81_ELISA_19011519_424.jpg

GTX54522 ELISA Image

Sandwich ELISA was performed with a Huntingtin monoclonal antibody and GTX54522 Huntingtin (neoepitope 586) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo586 in 293T cells overexpressing the Htt construct.

GTX54522_2084_WB_19011519_597.jpg

GTX54522 WB Image

WB analysis of endogenous HTT lysates with or without different caspase activity (Lanes 1-5) and overexpressed recombinant HTT fragment lysates (Lanes 6-9) using GTX54522 Huntingtin (neoepitope 586) antibody (Bottom panel) and pan-HTT antibody (Top panel).
Dilution : 1:500
Loading :20 µg

Package List Price ($)
$ 319