APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:4,000 |
| 1:2,000 |
| 1:2000 |
| 1:2000 |
| 1/50 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site. And it recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.
PROPERTIES
Form
Liquid
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Pig
Immunogen
Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ATPase H+/K+ transporting subunit beta , ATP6B
Cellular Localization
Cell membrane; Single-pass type II membrane protein
Background
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866](/templates/default/js/lightbox/images/loading.gif "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866")
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GTX22866 IHC-P Image
IHC-P analysis of human stomach tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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GTX22866 ICC/IF Image
ICC/IF analysis of A2058 Cells using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX22866 IHC-P Image
IHC-P analysis of human gastric carcinoma tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
|
REFERENCE
There are currently no references for Hydrogen Potassium ATPase Beta antibody [2G11] (GTX22866). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Hydrogen Potassium ATPase Beta antibody [2G11] (GTX22866). Be the first to share your experience with this product.
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866](/upload/website/prouct_img/small/GTX22866/GTX22866_1182_IHC-P_19011519_272.jpg "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866")
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22866](/upload/website/prouct_img/small/GTX22866/GTX22866_528_ICC-IF_19011519_189.jpg "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22866")
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866](/upload/website/prouct_img/small/GTX22866/GTX22866_1181_IHC-P_19011519_234.jpg "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866")
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866](/upload/website/prouct_img/normal/GTX22866/GTX22866_1182_IHC-P_19011519_272.jpg "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866")
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22866](/upload/website/prouct_img/normal/GTX22866/GTX22866_528_ICC-IF_19011519_189.jpg "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22866")
![Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866](/upload/website/prouct_img/normal/GTX22866/GTX22866_1181_IHC-P_19011519_234.jpg "Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866")