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Hydrogen Potassium ATPase Beta antibody [2G11]

Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866
Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866
Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22866

Cat. No. GTX22866

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Dog, Guinea pig, Pig, Mustelid, Rodent
Package
100 μl ($399)
See all Hydrogen Potassium ATPase Beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4,000
ICC/IF 1:2,000
IHC-P 1:2000
IHC-Fr 1:2000
FACS 1/50
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site. And it recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+/K+ transporting subunit beta , ATP6B

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866

GTX22866 IHC-P Image

IHC-P analysis of human gastric carcinoma tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in IHC (Paraffin sections) (IHC-P). GTX22866

GTX22866 IHC-P Image

IHC-P analysis of human stomach tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hydrogen Potassium ATPase Beta antibody [2G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22866

GTX22866 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

REFERENCE

Application Reference

Zhao Y et al. Biomedicines 2023; 11 (9) High Hepcidin Levels Promote Abnormal Iron Metabolism and Ferroptosis in Chronic Atrophic Gastritis.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399