GeneTex
  • Country / Location Selection

United States (US)

IARS antibody

Anti-IARS antibody used in Western Blot (WB). GTX131733
Anti-IARS antibody used in Immunoprecipitation (IP). GTX131733
Anti-IARS antibody used in IHC (Paraffin sections) (IHC-P). GTX131733

Cat No. GTX131733

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

144 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

isoleucyl-tRNA synthetase,GRIDHH,IARS1,ILERS,ILRS,IRS,PRO0785

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Database

DATA IMAGES
Anti-IARS antibody used in Western Blot (WB). GTX131733

GTX131733 WB Image

IARS antibody detects IARS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IARS antibody (GTX131733) diluted at 1:4000.

Anti-IARS antibody used in Immunoprecipitation (IP). GTX131733

GTX131733 IP Image

Immunoprecipitation of IARS protein from 293T whole cell extracts using 5 μg of IARS antibody (GTX131733).
Western blot analysis was performed using IARS antibody (GTX131733).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-IARS antibody used in IHC (Paraffin sections) (IHC-P). GTX131733

GTX131733 IHC-P Image

IARS antibody detects IARS protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
IARS antibody (GTX131733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
There are currently no references for IARS antibody (GTX131733). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IARS antibody (GTX131733). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169