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ICAD antibody

Anti-ICAD antibody used in Western Blot (WB). GTX74224

Cat. No. GTX74224

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μg ($479)
See all ICAD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2μg/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

This antibody recognizes non-cleaved (45kDa) and cleaved DFF.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 2-21 at the N-terminus of human DFF45 (accession no. NP_004392).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_377689

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DFF1 , DFF45 , DFFA , DNA fragmentation factor subunit alpha , ICAD

Cellular Localization

Cytoplasm

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ICAD antibody used in Western Blot (WB). GTX74224

GTX74224 WB Image

WB analysis of HeLa (H), K562 (K), Jurkat (J), and Raji (R) cell lysates using GTX74224 ICAD antibody.
Dilution : 2μg/ml

REFERENCE

Application Reference

Liu X et al. Cell 1997; 89 (2) : 175-84 DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.
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SDS
PBS.pdf
Package List Price ($)
$ 479