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ICAD (cleaved Asp224) antibody

Anti-ICAD (cleaved Asp224) antibody used in Western Blot (WB). GTX86959
Anti-ICAD (cleaved Asp224) antibody used in IHC (Paraffin sections) (IHC-P). GTX86959

Cat. No. GTX86959

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ICAD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

ICAD (cleaved Asp224) antibody detects endogenous levels of fragment of activated DFF45 protein resulting from cleavage adjacent to Asp224.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ICAD (175-224).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DFF1 , DFF45 , DFFA , DNA fragmentation factor subunit alpha , ICAD

Cellular Localization

Cytoplasm

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ICAD (cleaved Asp224) antibody used in Western Blot (WB). GTX86959

GTX86959 WB Image

WB analysis of 293 cell lysate using GTX86959 ICAD (cleaved Asp224) antibody.

Anti-ICAD (cleaved Asp224) antibody used in IHC (Paraffin sections) (IHC-P). GTX86959

GTX86959 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86959 ICAD (cleaved Asp224) antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399