Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Neat |
Note :
FACS
Use 20μl of the suggested working dilution to label 5 x 105 cells or 100μl of whole blood.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody detects an antigen of human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.
Form
Liquid
Buffer
PBS, 0.2% BSA
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
K562 cell line.
Purification
Purified by affinity chromatography
Conjugation
Fluorescein isothiocyanate (FITC)
RRID
AB_377254
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
intercellular adhesion molecule 1 , BB2 , CD54 , P3.58
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Database
Research Area