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ICAM1 / CD54 antibody [84H10]

Cat. No. GTX76273

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 84H10

Isotype

 IgG1

Application

 IHC-Fr, FACS, IP, ELISA, Neutralizing/Inhibition

Reactivity

 Human, Dog
Package
100 μg ($399)
See all ICAM1 / CD54 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr 1/20-1/50
FACS Neat-1/10
IP 5μg / 107 cells
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Product Note

This antibody detects an antigen of human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

K562 cell line.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_377253

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Clone Reference

Chang CY et al. Eur J Pharmacol 2021; 911:174416 Novel method to repair articular cartilage by direct reprograming of prechondrogenic mesenchymal stem cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399