ICAM1 / CD54 antibody [HL4447]

ICAM1 / CD54 antibody [HL4447] detects ICAM1 / CD54 protein by immunofluorescent analysis.
Sample: Ramos cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [HL4447] (GTX644012) diluted at 1:500.
Blue: Fluoroshield™ with DAPI (GTX30920).

ICAM1 / CD54 antibody [HL4447] detects ICAM1 / CD54 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
ICAM1 / CD54 stained by ICAM1 / CD54 antibody [HL4447] (GTX644012) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ICAM1 / CD54 antibody [HL4447] detects ICAM1 / CD54 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
ICAM1 / CD54 stained by ICAM1 / CD54 antibody [HL4447] (GTX644012) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [HL4447] (GTX644012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [HL4447] (GTX644012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ICAM1 / CD54 antibody [HL4447] detects ICAM1 / CD54 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human artery.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [HL4447] (GTX644012) diluted at 1:200.
Red: VE-Cadherin antibody [GT1369] (GTX633705) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min