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ICAM1 / CD54 antibody [N1C2]

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

Cat No. GTX100450

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

58 or 85~110 kDa. The observed M.W. is based on the following publication. PMID: 24474243

Positive Control

HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ICAM1 / CD54. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1 / CD54(GTX100450) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX100450 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

The WB analysis of ICAM1 / CD54 antibody [N1C2] was published by Lee TH and colleagues in the journal PLoS One in 2015.PMID: 25675397

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

The WB analysis of ICAM1 / CD54 antibody [N1C2] was published by Lee TH and colleagues in the journal PLoS One in 2015.PMID: 25675397

REFERENCE

REVIEW

ICAM1 / CD54 antibody [N1C2] Cat No. GTX100450

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3ug/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10ug/mL) MG63 whole cell lysate
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169