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ICAM1 / CD54 antibody [N1C2]

Orthogonal
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

Cat. No. GTX100450

Reference 10
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ICAM1 / CD54 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

58 or 85~110 kDa. The observed M.W. is based on the following publication. PMID: 24474243

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ICAM1 / CD54. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950536

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1 / CD54(GTX100450) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
Application : WB
Reactivity : Human
Chang WC et al. Plants (Basel) 2021; 10 (7) Vescalagin from Pink Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Protects Pancreatic β-Cells against Methylglyoxal-Induced Inflammation in Rats.
Application : WB
Reactivity : Rat
Corgnac S et al. Cell Rep Med 2020; 1 (7) : 100127 CD103+CD8+ TRM Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17.
Application : IHC-P
Reactivity : Human
Huang FI et al. Front Oncol 2019; 9 : 249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
Sol? M et al. Biochim Biophys Acta Mol Basis Dis 2019; (Epub) Blood-brain barrier dysfunction underlying Alzheimer's disease is induced by an SSAO/VAP-1-dependent cerebrovascular activation with enhanced Aβ deposition.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

ICAM1 / CD54 antibody [N1C2] Cat. No. GTX100450

Rating
( Average 5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 4 users reviews)
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25675397
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25675397
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3ug/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10ug/mL) MG63 whole cell lysate
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
Application : WB
Reactivity : Human
Chang WC et al. Plants (Basel) 2021; 10 (7) Vescalagin from Pink Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Protects Pancreatic β-Cells against Methylglyoxal-Induced Inflammation in Rats.
Application : WB
Reactivity : Rat
Corgnac S et al. Cell Rep Med 2020; 1 (7):100127 CD103+CD8+ TRM Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17.
Application : IHC-P
Reactivity : Human
Huang FI et al. Front Oncol 2019; 9:249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
Sol? M et al. Biochim Biophys Acta Mol Basis Dis 2019; (Epub) Blood-brain barrier dysfunction underlying Alzheimer's disease is induced by an SSAO/VAP-1-dependent cerebrovascular activation with enhanced Aβ deposition.
Application : WB
Reactivity : Human
Chang MC et al. J Formos Med Assoc 2018; (Epub) IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38.
Application : WB
Reactivity : Human
Sun P et al. Biochim Biophys Acta 2017; 1864 (2):542-553 Simvastatin blocks soluble SSAO/VAP-1 release in experimental models of cerebral ischemia: Possible benefits for stroke-induced inflammation control.
Application : WB
Reactivity : Human
Lee TH et al. PLoS One 2015; Collagen inhibitory peptide R1R2 mediates vascular remodeling by decreasing inflammation and smooth muscle cell activation.
Application : WB
Reactivity : Human
Claude S et al. Mol Nutr Food Res 2014; 58 (5):1016-27 Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
Application : WB
Reactivity : Human
Koya T et al. Am J Physiol Heart Circ Physiol 2012; 303 (1):H96-105 Salusin-β accelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169