GeneTex
  • Country / Location Selection

United States (US)

ICAM1 / CD54 antibody [N1C2]

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

Cat No. GTX100450

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

58 or 85~110 kDa. The observed M.W. is based on the following publication. PMID: 24474243

Positive Control

HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ICAM1 / CD54. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950536

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1 / CD54(GTX100450) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX100450 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ICAM1 / CD54 antibody [N1C2] Cat No. GTX100450

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25675397
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25675397
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3ug/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10ug/mL) MG63 whole cell lysate
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf