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ICAM1 / CD54 antibody [N1C2]

Orthogonal
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

Cat. No. GTX100450

Reference 14
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ICAM1 / CD54 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

58 or 85~110 kDa. The observed M.W. is based on the following publication. PMID: 24474243

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ICAM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950536

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1 / CD54(GTX100450) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25675397

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25675397

REFERENCE

Application Reference

YJ Shi et al. Eur J Pharmacol 2023; Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction
Vos S et al. Invest Ophthalmol Vis Sci 2023; 64 (7) : 17 CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression.
Wu CY et al. J Cell Mol Med 2023; 27 (11) : 1509-1522 CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells.
Chang TM et al. Int J Biol Sci 2023; 19 (5) : 1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : WB
Reactivity : Human
Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

ICAM1 / CD54 antibody [N1C2] Cat. No. GTX100450

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3ug/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10ug/mL) MG63 whole cell lysate
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
YJ Shi et al. Eur J Pharmacol 2023; Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction
Vos S et al. Invest Ophthalmol Vis Sci 2023; 64 (7):17 CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression.
Wu CY et al. J Cell Mol Med 2023; 27 (11):1509-1522 CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells.
Chang TM et al. Int J Biol Sci 2023; 19 (5):1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : WB
Reactivity : Human
Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
Application : WB
Reactivity : Human
Chang WC et al. Plants (Basel) 2021; 10 (7) Vescalagin from Pink Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Protects Pancreatic β-Cells against Methylglyoxal-Induced Inflammation in Rats.
Application : WB
Reactivity : Rat
Corgnac S et al. Cell Rep Med 2020; 1 (7):100127 CD103+CD8+ TRM Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17.
Application : IHC-P
Reactivity : Human
Huang FI et al. Front Oncol 2019; 9:249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
Sol? M et al. Biochim Biophys Acta Mol Basis Dis 2019; (Epub) Blood-brain barrier dysfunction underlying Alzheimer's disease is induced by an SSAO/VAP-1-dependent cerebrovascular activation with enhanced Aβ deposition.
Application : WB
Reactivity : Human
Chang MC et al. J Formos Med Assoc 2018; (Epub) IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38.
Application : WB
Reactivity : Human
Sun P et al. Biochim Biophys Acta 2017; 1864 (2):542-553 Simvastatin blocks soluble SSAO/VAP-1 release in experimental models of cerebral ischemia: Possible benefits for stroke-induced inflammation control.
Application : WB
Reactivity : Human
Lee TH et al. PLoS One 2015; Collagen inhibitory peptide R1R2 mediates vascular remodeling by decreasing inflammation and smooth muscle cell activation.
Application : WB
Reactivity : Human
Claude S et al. Mol Nutr Food Res 2014; 58 (5):1016-27 Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
Application : WB
Reactivity : Human
Koya T et al. Am J Physiol Heart Circ Physiol 2012; 303 (1):H96-105 Salusin-β accelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169