ICAM2 antibody [N1C3]

Cat. No. GTX118110

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($419), 25 μl ($169)

See all ICAM2 products

Datasheet
Document

View product citations for antibody GTX118110 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ICAM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616684

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intercellular adhesion molecule 2 , CD102

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3384 ICAM2
UniProt: P13598 ICAM2

Research Area

DATA IMAGES

GTX118110 IHC-P Image

ICAM2 antibody [N1C3] detects ICAM2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ICAM2 stained by ICAM2 antibody [N1C3] (GTX118110) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX118110 ICC/IF Image

ICAM2 antibody [N1C3] detects ICAM2 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ICAM2 protein stained by ICAM2 antibody [N1C3] (GTX118110) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX118110 WB Image

Sample (30 ug of whole cell lysate)
A: K562
7.5% SDS PAGE
GTX118110 diluted at 1:3000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 419

25 μl

$ 169

Qty