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ICAM3 antibody [186-2G9]

Anti-ICAM3 antibody [186-2G9] used in IHC (Paraffin sections) (IHC-P). GTX34789

Cat No. GTX34789

Host

Mouse

Clonality

Monoclonal

Clone Name

186-2G9

Isotype

IgG2b

Application

ICC/IF, IHC-P, Neutralizing/Inhibition

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
Neutralizing/Inhibition Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Neutralizing/Inhibition
Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3). Please contact us for PBS only format.

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Stimulated human leukocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD50 , CDW50 , ICAM3 , ICAMR , intercellular adhesion molecule 3

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ICAM3 antibody [186-2G9] used in IHC (Paraffin sections) (IHC-P). GTX34789

GTX34789 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34789 ICAM3 antibody [186-2G9].

REFERENCE

There are currently no references for ICAM3 antibody [186-2G9] (GTX34789). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ICAM3 antibody [186-2G9] (GTX34789). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339