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ICAM3 antibody [186-2G9]

Anti-ICAM3 antibody [186-2G9] used in IHC (Paraffin sections) (IHC-P). GTX34789

Cat. No. GTX34789

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 186-2G9

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($529)
See all ICAM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
Neutralizing/Inhibition Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Neutralizing/Inhibition
Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3). Please contact us for PBS only format.
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Stimulated human leukocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD50 , CDW50 , ICAM3 , ICAMR , intercellular adhesion molecule 3

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ICAM3 antibody [186-2G9] used in IHC (Paraffin sections) (IHC-P). GTX34789

GTX34789 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34789 ICAM3 antibody [186-2G9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529