ICAM3 antibody [ICAM3/1019]

Cat. No. GTX34791

Host	Mouse
Clonality	Monoclonal
Clone Name	ICAM3/1019
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

See all ICAM3 products

Datasheet
Document

View product citations for antibody GTX34791 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ICAM3 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD50 , CDW50 , ICAM3 , ICAMR , intercellular adhesion molecule 3

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Database

Gene ID: 3385 ICAM3
UniProt: P32942 ICAM3

Research Area

DATA IMAGES

GTX34791 WB Image

WB analysis of HL-60 cell lysate using GTX34791 ICAM3 antibody [ICAM3/1019].

GTX34791 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34791 ICAM3 antibody [ICAM3/1019].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty