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ICAM3 antibody [ICAM3/1019]

Anti-ICAM3 antibody [ICAM3/1019] used in Western Blot (WB). GTX34791
Anti-ICAM3 antibody [ICAM3/1019] used in IHC (Paraffin sections) (IHC-P). GTX34791

Cat No. GTX34791

Host

Mouse

Clonality

Monoclonal

Clone Name

ICAM3/1019

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ICAM3 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD50 , CDW50 , ICAM3 , ICAMR , intercellular adhesion molecule 3

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ICAM3 antibody [ICAM3/1019] used in Western Blot (WB). GTX34791

GTX34791 WB Image

WB analysis of HL-60 cell lysate using GTX34791 ICAM3 antibody [ICAM3/1019].

Anti-ICAM3 antibody [ICAM3/1019] used in IHC (Paraffin sections) (IHC-P). GTX34791

GTX34791 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34791 ICAM3 antibody [ICAM3/1019].

REFERENCE

There are currently no references for ICAM3 antibody [ICAM3/1019] (GTX34791). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ICAM3 antibody [ICAM3/1019] (GTX34791). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339