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ICAM3 antibody [ICAM3/1019]

Anti-ICAM3 antibody [ICAM3/1019] used in IHC (Paraffin sections) (IHC-P). GTX34791
Anti-ICAM3 antibody [ICAM3/1019] used in Western Blot (WB). GTX34791

Cat No. GTX34791

Host

Mouse

Clonality

Monoclonal

Clone Name

ICAM3/1019

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human ICAM3 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Intercellular Adhesion Molecule 3 , Cd50 , Cdw50 , Icam-R , Icam3

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ICAM3 antibody [ICAM3/1019] used in IHC (Paraffin sections) (IHC-P). GTX34791

GTX34791 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (ICAM3/1019)

Anti-ICAM3 antibody [ICAM3/1019] used in Western Blot (WB). GTX34791

GTX34791 WB Image

Western Blot Analysis of HL-60 Cell Lysate using CD50 Monoclonal Antibody (ICAM3/1019)

REFERENCE

There are currently no references for ICAM3 antibody [ICAM3/1019] (GTX34791). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ICAM3 antibody [ICAM3/1019] (GTX34791). Be the first to share your experience with this product.
Package List Price ($)
$ 429