GeneTex
United States (US)

ICAM3 antibody [ICAM3/1019]

GTX34791_20170712_IHC-P_18121410_559.jpg
GTX34791_20170712_WB_18121410_194.jpg

Cat No. GTX34791

Host Mouse
Clonality Monoclonal
Clone Name ICAM3/1019
Isotype IgG2b
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

WB:0.5-1ug/ml, IHC-P:0.5-1ug/ml for 30 minutes at RT.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human ICAM3 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Intercellular Adhesion Molecule 3,Cd50,Cdw50,Icam-R,Icam3

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34791_20170712_IHC-P_18121410_559.jpg

GTX34791 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (ICAM3/1019)

GTX34791_20170712_WB_18121410_194.jpg

GTX34791 WB Image

Western Blot Analysis of HL-60 Cell Lysate using CD50 Monoclonal Antibody (ICAM3/1019)

Package List Price ($)
$ 329