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ICOS Ligand antibody [HK5.3] (PE)

Anti-ICOS Ligand antibody [HK5.3] (PE) used in Flow cytometry (FACS). GTX01490-08

Cat. No. GTX01490-08

Reference 3

Host

 Rat

Clonality

 Monoclonal

Clone Name

 HK5.3

Isotype

 IgG2a

Application

 FACS, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($399)
See all ICOS Ligand products
PRODUCT

Summary

The HK5.3 antibody reacts with mouse CD275, an approximately 40 kDa protein that is a member of the Ig superfamily. CD275 is also known as B7-H2, ICOS ligand, and B7-RP1. CD275 is a ligand for ICOS (CD278) that is expressed on activated T cells. CD275 is expressed by antigen presenting cells including B cells, macrophages, and dendritic cells. The interaction of CD275 with its receptor ICOS plays an important role in T cell costimulation.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-ICOS Ligand antibody [HK5.3] (PE) used in Flow cytometry (FACS). GTX01490-08

GTX01490-08 FACS Image

FACS analysis of mouse C57Bl/6 spleen cells using GTX01490-08 ICOS Ligand antibody [HK5.3] (PE).
Right panel : co-stained with ICOS Ligand antibody [HK5.3] (PE) and Mouse CD3 antibody (APC)
Left panel : co-stained with isotype control and Mouse CD3 antibody (APC)
antibody amount : 0.06 μg (5 μl)

REFERENCE

Clone Reference

2004; Preventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders.
Application : Neutralizing/Inhibition
Reactivity : Mouse
2004; Inducible costimulator-dependent IL-10 production by regulatory T cells specific for self-antigen.
2003; Involvement of inducible costimulator-B7 homologous protein costimulatory pathway in murine lupus nephritis.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399