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ID1 antibody

Anti-ID1 antibody used in Western Blot (WB). GTX133738
Anti-ID1 antibody used in Western Blot (WB). GTX133738
Anti-ID1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133738
Anti-ID1 antibody used in Western Blot (WB). GTX133738
Anti-ID1 antibody used in Western Blot (WB). GTX133738

Cat No. GTX133738

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

A431 , HeLa , HepG2 , mouse ESC , ID1-transfected 293T

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse ID1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887087

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inhibitor of DNA binding 1 , AI323524 , D2Wsu140e , Idb1 , bHLHb24

Database

Research Area

DATA IMAGES

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with ID1 antibody (GTX133738) diluted at 1:500 and competitor's antibody (sc-488) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ID1 antibody (GTX133738) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ID1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133738

GTX133738 ICC/IF Image

ID1 antibody detects ID1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ID1 stained by ID1 antibody (GTX133738) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ID1 antibody (GTX133738) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ID1 antibody (GTX133738) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf