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ID1 antibody

Anti-ID1 antibody used in IHC (Paraffin sections) (IHC-P). GTX53624
Anti-ID1 antibody used in Western Blot (WB). GTX53624
Anti-ID1 antibody used in Western Blot (WB). GTX53624
Anti-ID1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53624
Anti-ID1 antibody used in Flow cytometry (FACS). GTX53624

Cat. No. GTX53624

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
400 μl ($399)
See all ID1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 1, HLH protein , ID , bHLHb24

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ID1 antibody used in IHC (Paraffin sections) (IHC-P). GTX53624

GTX53624 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX53624 ID1 antibody.

Anti-ID1 antibody used in Western Blot (WB). GTX53624

GTX53624 WB Image

WB analysis of U251 cell lysate (35ug/lane) using GTX53624 ID1 antibody.

Anti-ID1 antibody used in Western Blot (WB). GTX53624

GTX53624 WB Image

WB analysis of mouse heart tissue lysate (35ug/lane) using GTX53624 ID1 antibody.
Dilution : 1:1000

Anti-ID1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53624

GTX53624 ICC/IF Image

ICC/IF analysis of U251 cells using GTX53624 ID1 antibody.
Green : ID1
Blue : DAPI

Anti-ID1 antibody used in Flow cytometry (FACS). GTX53624

GTX53624 FACS Image

FACS analysis of HepG2 cells using GTX53624 ID1 antibody.
Green : primary antibodyID1
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399