GeneTex
United States (US)

ID1 antibody

GTX53624_1620_WB_18121410_313.jpg
GTX53624_761_IHC-P_18121410_560.jpg
GTX53624_1619_WB_18121410_671.jpg
GTX53624_577_ICC-IF_18121410_616.jpg
GTX53624_65_FACS_18121410_898.jpg

Cat No. GTX53624

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

16 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Inhibitor Of Dna Binding 1, Hlh Protein,Id,Bhlhb24,Id1

Background

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX53624_1620_WB_18121410_313.jpg

GTX53624 WB Image

WB analysis of mouse heart tissue lysate (35ug/lane) using GTX53624 ID1 antibody.
Dilution : 1:1000

GTX53624_761_IHC-P_18121410_560.jpg

GTX53624 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX53624 ID1 antibody.

GTX53624_1619_WB_18121410_671.jpg

GTX53624 WB Image

WB analysis of U251 cell lysate (35ug/lane) using GTX53624 ID1 antibody.

GTX53624_577_ICC-IF_18121410_616.jpg

GTX53624 ICC/IF Image

ICC/IF analysis of U251 cells using GTX53624 ID1 antibody.
Green : ID1
Blue : DAPI

GTX53624_65_FACS_18121410_898.jpg

GTX53624 FACS Image

FACS analysis of HepG2 cells using GTX53624 ID1 antibody.
Green : primary antibodyID1
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25