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ID2 antibody [N1C3]

Anti-ID2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109113
Anti-ID2 antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX109113
Anti-ID2 antibody [N1C3] used in Western Blot (WB). GTX109113

Cat. No. GTX109113

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ID2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037216

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 2 , GIG8 , ID2A , ID2H , bHLHb26

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ID2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109113

GTX109113 IHC-P Image

ID2 antibody [N1C3] detects ID2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ID2 antibody [N1C3] (GTX109113) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ID2 antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX109113

GTX109113 IHC-Fr Image

ID2 antibody [N1C3] detects ID2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: ID2 stained by ID2 antibody [N1C3] (GTX109113) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ID2 antibody [N1C3] used in Western Blot (WB). GTX109113

GTX109113 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
15% SDS PAGE
GTX109113 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169