GeneTex

  • Country / Location Selection

United States (US)

ID3 antibody [2B11]

Anti-ID3 antibody [2B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70201
Anti-ID3 antibody [2B11] used in Western Blot (WB). GTX70201

Cat. No. GTX70201

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B11

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ID3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 5 μg/ml
ICC/IF 1:100-1:1000
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

Id3-transfected 293T , Id3 transfected Sol8 muscle cells and recombinant fusion protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The full-length (amino acids 1-119) mouse Id3 gene expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_371881

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 3 , Hlh462 , Idb3 , bHLHb25

Cellular Localization

Nucleus , Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ID3 antibody [2B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70201

GTX70201 ICC/IF Image

Id3 antibody [2B11] detects Id3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Id3 protein stained by Id3 antibody [2B11] (GTX70201) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ID3 antibody [2B11] used in Western Blot (WB). GTX70201

GTX70201 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] (GTX70201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for ID3 antibody [2B11] (GTX70201). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ID3 antibody [2B11] (GTX70201). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations