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ID3 antibody [2B11]

Anti-ID3 antibody [2B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70201
Anti-ID3 antibody [2B11] used in Western Blot (WB). GTX70201

Cat No. GTX70201

Host

Mouse

Clonality

Monoclonal

Clone Name

2B11

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 5 μg/ml
ICC/IF 1:100-1:1000
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Id3-transfected 293T , Id3 transfected Sol8 muscle cells and recombinant fusion protein

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The full-length (amino acids 1-119) mouse Id3 gene expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Inhibitor Of Dna Binding 3 , Hlh462 , Idb3 , Bhlhb25 , Id3

Background

Id3-2B11 recognizes Id3, a 14a protein that is a member of the Id family of helix-loop-helix proteins. Id proteins are believed to be dominant negative regulators of bHLH proteins involved in cell differentiation and implicated in the control of cell growth, cellular differentiation, apoptosis and carcinogenesis.

Database

Research Area

DATA IMAGES

Anti-ID3 antibody [2B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70201

GTX70201 ICC/IF Image

Id3 antibody [2B11] detects Id3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Id3 protein stained by Id3 antibody [2B11] (GTX70201) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ID3 antibody [2B11] used in Western Blot (WB). GTX70201

GTX70201 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] (GTX70201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329