GeneTex
United States (US)

IDE antibody [N1N3]

KO/KD
GTX105095_40366_WB_19011506_294.jpg
GTX105095_40366_20170309_WB_KO_watermark_19011506_133.jpg

Cat No. GTX105095

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ35968 antibody, INSULYSIN antibody, IDE antibody, insulinase antibody, Abeta-degrading protease antibody, insulin protease antibody, insulin-degrading enzyme antibody

Background

This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105095_40366_WB_19011506_294.jpg

GTX105095 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX105095 diluted at 1:1000

GTX105095_40366_20170309_WB_KO_watermark_19011506_133.jpg

GTX105095 WB Image

KO/KD

Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N1N3] (GTX105095) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169