GeneTex

  • Country / Location Selection

United States (US)

IDE antibody [N3C1], Internal

KO/KD Orthogonal
Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664
Anti-IDE antibody [N3C1], Internal used in IHC (Whole mount) (IHC-Wm). GTX111664
Anti-IDE antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111664
Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664
Anti-IDE antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111664
Anti-IDE antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111664
Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664
Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664

Cat. No. GTX111664

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all IDE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950539

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin degrading enzyme , INSULYSIN

Cellular Localization

Cytoplasm

Background

This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664

GTX111664 WB Image

KO/KD

Wild-type (WT) and IDE Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IDE antibody [N3C1], Internal used in IHC (Whole mount) (IHC-Wm). GTX111664

GTX111664 IHC-Wm Image

IDE antibody [N3C1], Internal detects Ide protein at the myotome on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
IDE antibody [N3C1], Internal (GTX111664) dilution: 1:200.

Anti-IDE antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111664

GTX111664 IHC-P Image

IDE antibody [N3C1], Internal detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
IDE antibody [N3C1], Internal (GTX111664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664

GTX111664 WB Image

Various extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IDE antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111664

GTX111664 ICC/IF Image

IDE antibody [N3C1], Internal detects IDE protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: IDE stained by IDE antibody [N3C1], Internal (GTX111664) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IDE antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111664

GTX111664 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using insulin degrading enzyme(GTX111664) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664

GTX111664 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IDE antibody [N3C1], Internal used in Western Blot (WB). GTX111664

GTX111664 WB Image

Orthogonal

The data was published in the journal PLoS One in 2018.PMID: 29715265

REFERENCE

Application Reference

Hamilton KA et al. PLoS One 2018; 13 (5) : e0196223 Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for IDE antibody [N3C1], Internal (GTX111664). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169