APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:2000 |
| 1:10-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.02% sodium azide, 50% glycerol.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
insulin degrading enzyme , INSULYSIN
Cellular Localization
Cytoplasm,Cell membrane,Secreted
Background
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
|
GTX32665 WB Image
WB analysis of various sample lysates using GTX32665 IDE antibody.
Loading : 25ug per lane
Dilution : 1:1000
|
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GTX32665 WB Image
WB analysis of normal (control) and IDE knockout (KO) HeLa cell lysate using GTX32665 IDE antibody.
Loading : 25ug per lane
Dilution : 1:1000
|
|
GTX32665 ICC/IF Image
ICC/IF analysis of A549 cells using GTX32665 IDE antibody.
Blue : DAPI
|
REFERENCE
There are currently no references for IDE antibody (GTX32665). Be the first to share your publications with this product.
REVIEW
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