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IDE antibody

Anti-IDE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32665
Anti-IDE antibody used in Western Blot (WB). GTX32665
Anti-IDE antibody used in Western Blot (WB). GTX32665

Cat No. GTX32665

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin degrading enzyme , INSULYSIN

Cellular Localization

Cytoplasm,Cell membrane,Secreted

Background

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-IDE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32665

GTX32665 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32665 IDE antibody.
Blue : DAPI

Anti-IDE antibody used in Western Blot (WB). GTX32665

GTX32665 WB Image

WB analysis of wild type and IDE knockout cell lysate using GTX32665 IDE antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IDE antibody used in Western Blot (WB). GTX32665

GTX32665 WB Image

WB analysis of various samples using GTX32665 IDE antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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