IDH1 (R132H Mutant) antibody [HL4884]

IDH1 (R132H Mutant) antibody [HL4884] detects IDH1 (R132H Mutant) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde.
Green: IDH1 (R132H Mutant) stained by IDH1 (R132H Mutant) antibody [HL4884] (GTX644949) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

IDH1 (R132H Mutant) antibody [HL4884] detects IDH1 (R132H Mutant) protein by immunohistochemical analysis.
Sample: Mock, IDH1 (WT) and IDH1 (R132H Mutant) transfected 293T cell FFPE Cell Pellet Blocks.
Green: IDH1 (R132H Mutant) stained by IDH1 (R132H Mutant) antibody [HL4884] (GTX644949) diluted at 1:1000.
Red: DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IDH1 (R132H Mutant) antibody [HL4884] detects IDH1 (R132H Mutant) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
IDH1 (R132H Mutant) stained by IDH1 (R132H Mutant) antibody [HL4884] (GTX644949) diluted at 1:2500.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 (R132H Mutant) antibody [HL4884] (GTX644949) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.