APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT25H10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IDH3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
isocitrate dehydrogenase (NADP(+)) 1 , HEL216 , HELS26 , IDCD , IDH , IDH1 , IDP , IDPC , PICD , isocitrate dehydrogenase (NADP(+)) 1, cytosolic
Cellular Localization
Cytoplasm,Peroxisome
Background
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Database
Research Area
DATA IMAGES
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GTX57662 ICC/IF Image
ICC/IF analysis of Hep3B cells using GTX57662 IDH1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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GTX57662 WB Image
WB analysis of various samples using GTX57662 IDH1 antibody. Lane 1 : HepG2 whole cell lysate Lane 2 : A549 whole cell lysate Lane 3 : HeLa whole cell lysate Lane 4 : NIH-3T3 whole cell lysate Lane 5 : Jurkat whole cell lysate Lane 6 : K562 whole cell lysate Lane 7 : 293T whole cell lysate Lane 8 : MCF-7 whole cell lysate Lane 9 : LNCap whole cell lysate Dilution : 1:1000
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REFERENCE
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REVIEW
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