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IDH1 antibody [GT1521]

KO/KD Comparison Overexpression
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Immunoprecipitation (IP). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629818

Cat. No. GTX629818

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1521

Isotype

 IgG2b

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IDH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat-2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Huh-7

Predict Reactivity

Zebrafish, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888166

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

isocitrate dehydrogenase (NADP(+)) 1, cytosolic , HEL-216 , HEL-S-26 , IDCD , IDH , IDP , IDPC , PICD

Cellular Localization

Cytoplasm , Peroxisome

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:4000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

KO/KD

Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:1000 and competitor's antibody (CST#8137) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
IDH1 antibody [GT1521] (GTX629818) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
IDH1 antibody [GT1521] (GTX629818) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Immunoprecipitation (IP). GTX629818

GTX629818 IP Image

IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody (GTX629818) 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody (GTX629818) diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
IDH1 antibody [GT1521] (GTX629818) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629818

GTX629818 ICC/IF Image

IDH1 antibody [GT1521] detects IDH1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IDH1 protein stained by IDH1 antibody [GT1521] (GTX629818) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations