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IDH1 antibody [GT1521]

KO/KD Comparison Overexpression
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818
Anti-IDH1 antibody [GT1521] used in Immunoprecipitation (IP). GTX629818

Cat. No. GTX629818

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1521

Isotype

 IgG2b

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IDH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat-2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Huh-7

Predict Reactivity

Zebrafish, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888166

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isocitrate dehydrogenase (NADP(+)) 1, cytosolic , HEL-216 , HEL-S-26 , IDCD , IDH , IDP , IDPC , PICD

Cellular Localization

Cytoplasm , Peroxisome

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:4000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

KO/KD

Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:1000 and competitor's antibody (CST#8137) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
IDH1 antibody [GT1521] (GTX629818) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
IDH1 antibody [GT1521] (GTX629818) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629818

GTX629818 ICC/IF Image

IDH1 antibody [GT1521] detects IDH1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IDH1 protein stained by IDH1 antibody [GT1521] (GTX629818) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
IDH1 antibody [GT1521] (GTX629818) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Western Blot (WB). GTX629818

GTX629818 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (GTX629818) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH1 antibody [GT1521] used in Immunoprecipitation (IP). GTX629818

GTX629818 IP Image

IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody (GTX629818) 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody (GTX629818) diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

REFERENCE

Application Reference

Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations