GeneTex
United States (US)

IDH1 antibody

Anti-IDH1 antibody used in Western Blot (WB). GTX31417

Cat No. GTX31417

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 Cell Lysate(GTX27900)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IDH1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human IDH1.The immunogen is located within the last 50 amino acids of IDH1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Isocitrate Dehydrogenase (Nadp(+)) 1, Cytosolic,Hel-216,Hel-S-26,Idcd,Idh,Idp,Idpc,Picd,Idh1

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES
Anti-IDH1 antibody used in Western Blot (WB). GTX31417

GTX31417 WB Image

WB analysis of HepG2 cell lysate using GTX31417 IDH1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml