APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
rat brain , Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse brain , DDDDK-tagged IDH2-transfected 293T , HepG2
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888040
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH
Cellular Localization
Mitochondrion
Background
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX628487 IHC-P Image
IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. IDH2 antibody [GT673] (GTX628487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628487 IHC-P Image
IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. IDH2 antibody [GT673] (GTX628487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628487 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628487 WB Image
IDH2 antibody [GT673] detects IDH2 protein by western blot analysis. A. 50 μg mouse brain lysate/extract 10% SDS-PAGE IDH2 antibody [GT673] (GTX628487) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628487 ICC/IF Image
IDH2 antibody [GT673] detects IDH2 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] (GTX628487) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX628487 WB Image
IDH2 antibody detects IDH2 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg K562 whole cell lysate/extract D. 30 μg THP-1 whole cell lysate/extract E. 30 μg NCI-H929 whole cell lysate/extract 10% SDS-PAGE IDH2 antibody (GTX628487) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628487 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628487 ICC/IF Image
IDH2 antibody detects IDH2 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody (GTX628487) diluted at 1:500. Red: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX628487 WB Image
IDH2 antibody [GT673] detects IDH2 protein by western blot analysis. A. 50 μg rat brain lysate/extract 12% SDS-PAGE IDH2 antibody [GT673] (GTX628487) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
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