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IDH2 antibody [GT673]

KO/KD Overexpression
Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487
Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

Cat. No. GTX628487

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT673

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

rat brain , Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse brain , DDDDK-tagged IDH2-transfected 293T , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888040

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH

Cellular Localization

Mitochondrion

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487

GTX628487 IHC-P Image

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
IDH2 antibody [GT673] (GTX628487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487

GTX628487 IHC-P Image

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IDH2 antibody [GT673] (GTX628487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody [GT673] detects IDH2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
IDH2 antibody [GT673] (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

GTX628487 ICC/IF Image

IDH2 antibody [GT673] detects IDH2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: IDH2 protein stained by IDH2 antibody [GT673] (GTX628487) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody detects IDH2 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg NCI-H929 whole cell lysate/extract
10% SDS-PAGE
IDH2 antibody (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

GTX628487 ICC/IF Image

IDH2 antibody detects IDH2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: IDH2 protein stained by IDH2 antibody (GTX628487) diluted at 1:500.
Red: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody [GT673] detects IDH2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12% SDS-PAGE
IDH2 antibody [GT673] (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu TF et al. J Biol Chem 2015; 290 (1) : 396-408 Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations