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IDH2 antibody [GT673]

KO/KD Overexpression
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487
Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

Cat. No. GTX628487

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT673

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

rat brain , Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse brain , DDDDK-tagged IDH2-transfected 293T , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888040

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH

Cellular Localization

Mitochondrion

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody [GT673] detects IDH2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
IDH2 antibody [GT673] (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

GTX628487 ICC/IF Image

IDH2 antibody detects IDH2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: IDH2 protein stained by IDH2 antibody (GTX628487) diluted at 1:500.
Red: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody detects IDH2 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg NCI-H929 whole cell lysate/extract
10% SDS-PAGE
IDH2 antibody (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487

GTX628487 IHC-P Image

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
IDH2 antibody [GT673] (GTX628487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487

GTX628487 IHC-P Image

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IDH2 antibody [GT673] (GTX628487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody [GT673] detects IDH2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12% SDS-PAGE
IDH2 antibody [GT673] (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

GTX628487 ICC/IF Image

IDH2 antibody [GT673] detects IDH2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: IDH2 protein stained by IDH2 antibody [GT673] (GTX628487) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Liu TF et al. J Biol Chem 2015; 290 (1) : 396-408 Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations