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IDH2 antibody

Anti-IDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32666
Anti-IDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32666
Anti-IDH2 antibody used in ChIP assay (ChIP assay). GTX32666
Anti-IDH2 antibody used in Western Blot (WB). GTX32666
Anti-IDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32666
Anti-IDH2 antibody used in Immunoprecipitation (IP). GTX32666

Cat. No. GTX32666

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all IDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
ChIP assay 1:20 - 1:100
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH

Cellular Localization

Mitochondrion

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-IDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32666

GTX32666 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32666 IDH2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-IDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32666

GTX32666 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX32666 IDH2 antibody.
Dilution : 1:100

Anti-IDH2 antibody used in ChIP assay (ChIP assay). GTX32666

GTX32666 ChIP assay Image

ChIP analysis of 293T cell lysate using GTX32666 IDH2 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IDH2 antibody used in Western Blot (WB). GTX32666

GTX32666 WB Image

WB analysis of various sample lysates using GTX32666 IDH2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32666

GTX32666 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32666 IDH2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-IDH2 antibody used in Immunoprecipitation (IP). GTX32666

GTX32666 IP Image

IP analysis of MCF-7 cell lysate using GTX32666 IDH2 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419