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IDH3A antibody [2F11]

Overexpression
Anti-IDH3A antibody [2F11] used in Flow cytometry (FACS). GTX84317
Anti-IDH3A antibody [2F11] used in Western Blot (WB). GTX84317
Anti-IDH3A antibody [2F11] used in Western Blot (WB). GTX84317
Anti-IDH3A antibody [2F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84317
Anti-IDH3A antibody [2F11] used in Flow cytometry (FACS). GTX84317
Anti-IDH3A antibody [2F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84317

Cat. No. GTX84317

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F11

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Dog
Package
100 μl ($519)
See all IDH3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isocitrate dehydrogenase 3 (NAD(+)) alpha

Cellular Localization

Mitochondrion

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH3A antibody [2F11] used in Flow cytometry (FACS). GTX84317

GTX84317 FACS Image

FACS analysis of Jurkat cells using GTX84317 IDH3A antibody [2F11].
Red : Primary antibody
Blue : Negative control antibody

Anti-IDH3A antibody [2F11] used in Western Blot (WB). GTX84317

GTX84317 WB Image

WB analysis of various cell lines using GTX84317 IDH3A antibody [2F11].
Loading : 35 ug per lane

Anti-IDH3A antibody [2F11] used in Western Blot (WB). GTX84317

GTX84317 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IDH3A plasmid (Right) or empty vector (Left) for 48 hrs using GTX84317 IDH3A antibody [2F11].
Loading : 5 ug per lane

Anti-IDH3A antibody [2F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84317

GTX84317 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IDH3A plasmid using GTX84317 IDH3A antibody [2F11].

Anti-IDH3A antibody [2F11] used in Flow cytometry (FACS). GTX84317

GTX84317 FACS Image

FACS analysis of HeLa cells using GTX84317 IDH3A antibody [2F11].
Red : Primary antibody
Blue : Negative control antibody

Anti-IDH3A antibody [2F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84317

GTX84317 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84317 IDH3A antibody [2F11].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519