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IDO1 antibody [GT273]

Comparison Orthogonal Overexpression
Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652
Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652
Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652
Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652
Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652

Cat. No. GTX634652

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT273

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IDO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

IDO1-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human IDO1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888472

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

indoleamine 2,3-dioxygenase 1 , IDO , IDO-1 , INDO

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652

GTX634652 WB Image

Comparison Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IDO1 antibody [GT273] (GTX634652) diluted at 1:5000 and competitor's antibody (CST#12006) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652

GTX634652 IHC-P Image

IDO1 antibody [GT273] detects IDO1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
IDO1 stained by IDO1 antibody [GT273] (GTX634652) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652

GTX634652 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDO1 antibody [GT273] (GTX634652) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652

GTX634652 IHC-P Image

IDO1 antibody [GT273] detects IDO1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse mammary gland.
IDO1 stained by IDO1 antibody [GT273] (GTX634652) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652

GTX634652 IHC-P Image

IDO1 antibody [GT273] detects IDO1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
IDO1 stained by IDO1 antibody [GT273] (GTX634652) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Xu C et al. Stem Cell Res Ther 2022; 13 (1) : 491 TNFα and IFNγ rapidly activate PI3K-AKT signaling to drive glycolysis that confers mesenchymal stem cells enhanced anti-inflammatory property.
Sorrentino C et al. Front Immunol 2021; 12 : 778329 Colorectal Cancer-Associated Immune Exhaustion Involves T and B Lymphocytes and Conventional NK Cells and Correlates With a Shorter Overall Survival.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169