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IDO1 antibody [GT273]

Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652
Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652
Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652

Cat No. GTX634652

Host

Mouse

Clonality

Monoclonal

Clone Name

GT273

Isotype

IgG2a

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549 (100ng/mL IFN-gamma treatment for 48 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human IDO1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888472

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

indoleamine 2,3-dioxygenase 1 , IDO , IDO-1 , INDO

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652

GTX634652 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IDO1 antibody [GT273] (GTX634652) diluted at 1:5000 and competitor's antibody (CST#12006) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IDO1 antibody [GT273] used in IHC (Paraffin sections) (IHC-P). GTX634652

GTX634652 IHC-P Image

IDO1 antibody [GT273] detects IDO1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse mammary gland.
IDO1 stained by IDO1 antibody [GT273] (GTX634652) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDO1 antibody [GT273] used in Western Blot (WB). GTX634652

GTX634652 WB Image

A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDO1 antibody [GT273] (GTX634652) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for IDO1 antibody [GT273] (GTX634652). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IDO1 antibody [GT273] (GTX634652). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169