APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89419-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CVLANWKKKDPNK, from the internal region of the protein sequence according to NP_002155.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
indoleamine 2,3-dioxygenase 1 , IDO , IDO-1 , INDO
Cellular Localization
Cytoplasm, cytosol
Background
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
|
GTX89419 IHC-P Image
IHC-P analysis of human colon using GTX89419 IDO1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
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GTX89419 WB Image
WB analysis of human lymph node (A), spleen (B) and placenta (C) lysates using GTX89419 IDO1 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for IDO1 antibody, Internal (GTX89419). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IDO1 antibody, Internal (GTX89419). Be the first to share your experience with this product.
