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IDO1 antibody

Comparison Orthogonal Overexpression
Anti-IDO1 antibody used in Western Blot (WB). GTX113753
Anti-IDO1 antibody used in Western Blot (WB). GTX113753

Cat. No. GTX113753

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all IDO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549 (starvation for 24 hr and 100 ng/mL IFN-gamma treatment for 48 hr) , IDO1-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036477

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

indoleamine 2,3-dioxygenase 1 , IDO , IDO-1 , INDO

Background

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IDO1 antibody used in Western Blot (WB). GTX113753

GTX113753 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IDO1 antibody (GTX113753) diluted at 1:3000 and competitor's antibody (CST#12006S) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IDO1 antibody used in Western Blot (WB). GTX113753

GTX113753 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDO1 antibody (GTX113753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Takumi Yokosawa et al. Genes (Basel) 2020; 11 (12) Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells.
Application : WB
Reactivity : Human
Sreevalsan S et al. EMBO Rep 2020; 21 (12) : e50155 MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.
Gualdoni GS et al. Sci Rep 2019; 9 (1) : 15919 Role of indoleamine 2,3-dioxygenase in testicular immune-privilege.
Application : IHC-Fr
Reactivity : Rat
Wang LT et al. Cancer Res 2017; (Epub) Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan catabolism and immune suppression.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169