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IDS antibody, Internal

Anti-IDS antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88566
Anti-IDS antibody, Internal used in Western Blot (WB). GTX88566

Cat. No. GTX88566

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all IDS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Placenta shows lysosomal staining of trophoblasts.
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88566-PEP).

Product Note

This antibody is expected to recognize isoform a (NP_000193.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KHFRFRDLEEDP, from the internal region of the protein sequence according to NP_000193.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

iduronate 2-sulfatase , MPS2 , SIDS

Cellular Localization

Lysosome

Background

This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-IDS antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88566

GTX88566 IHC-P Image

IHC-P analysis of human placenta using GTX88566 IDS antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-IDS antibody, Internal used in Western Blot (WB). GTX88566

GTX88566 WB Image

WB analysis of human liver lysate using GTX88566 IDS antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399