GeneTex
United States (US)

IFIT2 antibody [N1], N-term

GTX108346_39904_20150410_IFA_19011507_288.jpg
GTX108346_WB_19011507_534.jpg

Cat No. GTX108346

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IFIT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interferon Induced Protein With Tetratricopeptide Repeats 2,G10P2,Garg-39,Ifi-54,Ifi-54K,Ifi54,Ifit-2,Isg-54 K,Isg-54K,Isg54,P54,Cig42,Ifit2

Database

Research Area

DATA IMAGES
GTX108346_39904_20150410_IFA_19011507_288.jpg

GTX108346 ICC/IF Image

IFIT2 antibody [N1], N-term detects IFIT2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term (GTX108346) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX108346_WB_19011507_534.jpg

GTX108346 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX108346 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169