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IFIT3 antibody

Orthogonal
Anti-IFIT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112442
Anti-IFIT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX112442
Anti-IFIT3 antibody used in Western Blot (WB). GTX112442
Anti-IFIT3 antibody used in Western Blot (WB). GTX112442
Anti-IFIT3 antibody used in Western Blot (WB). GTX112442

Cat. No. GTX112442

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IFIT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IFIT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950548

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon induced protein with tetratricopeptide repeats 3 , CIG-49 , GARG-49 , IFI60 , IFIT4 , IRG2 , ISG60 , P60 , RIG-G , cig41

Database

Research Area

DATA IMAGES

Anti-IFIT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112442

GTX112442 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IFIT3(GTX112442) antibody at 1:500 dilution.

Anti-IFIT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX112442

GTX112442 IHC-P Image

IFIT3 antibody detects IFIT3 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
IFIT3 antibody (GTX112442) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IFIT3 antibody used in Western Blot (WB). GTX112442

GTX112442 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody (GTX112442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IFIT3 antibody used in Western Blot (WB). GTX112442

GTX112442 WB Image

Orthogonal

A549 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody (GTX112442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IFIT3 antibody used in Western Blot (WB). GTX112442

GTX112442 WB Image

Orthogonal

The data was published in the 2020 in PLoS One. PMID: 32374750

REFERENCE

Application Reference

Chikhalya A et al. mBio 2021; : e0282921 Human IFIT3 Protein Induces Interferon Signaling and Inhibits Adenovirus Immediate Early Gene Expression.
Application : ICC/IF
Reactivity : Human
Imaizumi T et al. Kidney Blood Press Res 2021; 46 (1) : 74-83 IFIT Proteins Are Involved in CXCL10 Expression in Human Glomerular Endothelial Cells Treated with a Toll-Like Receptor 3 Agonist.
Application : WB
Reactivity : Human
Besson B et al. PLoS One 2020; 15 (5) : e0232585 Comparison of a human neuronal model proteome upon Japanese encephalitis or West Nile Virus infection and potential role of mosquito saliva in neuropathogenesis.
Application : WB
Reactivity : Human
Ferraris P et al. Emerg Microbes Infect 2019; 8 (1) : 1003-1016 Zika virus differentially infects human neural progenitor cells according to their state of differentiation and dysregulates neurogenesis through the Notch pathway.
Application : WB
Reactivity : Human
Imaizumi T et al. J Neuroimmunol 2018; 324 : 16-21 *-stimulated gene 60 (isg60) constitutes a negative feedback loop in the downstream of tlr3 signaling in hcmec/d3 cells.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169