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IFITM1 antibody

Orthogonal
Anti-IFITM1 antibody used in Western Blot (WB). GTX101728
Anti-IFITM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101728
Anti-IFITM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101728

Cat. No. GTX101728

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IFITM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IFITM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176549

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon induced transmembrane protein 1 , 9-27 , CD225 , DSPA2a , IFI17 , LEU13

Cellular Localization

Membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-IFITM1 antibody used in Western Blot (WB). GTX101728

GTX101728 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IFITM1 antibody (GTX101728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IFITM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101728

GTX101728 IHC-P Image

IFITM1 antibody detects IFITM1 protein at cytosol and membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
IFITM1 antibody (GTX101728) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IFITM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101728

GTX101728 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IFITM1(GTX101728) antibody at 1:500 dilution.

REFERENCE

Application Reference

Imaizumi T et al. J Neuroimmunol 2019; 337 : 577047 Interferon-induced transmembrane protein 1 and Myxovirus resistance protein 1 are induced by polyinosinic-polycytidylic acid in cultured hCMEC/D3 human cerebral microvascular endothelial cells.
Koh YW et al. Pathol Res Pract 2019; 215 (7) : 152444 Prognostic significance of IFITM1 expression and correlation with microvessel density and epithelial-mesenchymal transition signature in lung adenocarcinoma.
Application : IHC-P
Reactivity : Human
Yang Y. G. et al. IJC 2018; (Epub) *-induced transmembrane protein 1-mediated egfr/sox2 signaling axis is essential for progression of non-small cell lung cancer.
Application : WB
Reactivity : Human
Wu X et al. Cell 2017; (Epub) Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169