Western blot of immunoprecipitates from HEK 293 cells transfected with
1. Mock
2. IFNAR1 WT and
3. IFNAR1 Ser 535 Arg and Ser 539 Arg mutants.
The immunolabeling is absent in the IFNAR1 Ser 535 and Ser 539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).