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IFT80 antibody

Anti-IFT80 antibody used in IHC (Paraffin sections) (IHC-P). GTX109393
Anti-IFT80 antibody used in Western Blot (WB). GTX109393
Anti-IFT80 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109393

Cat No. GTX109393

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IFT80. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037306

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

intraflagellar transport 80 , ATD2 , SRTD2 , WDR56

Cellular Localization

Cytoplasm

Background

The IFT80 gene encodes a protein with 7 WD40 domains that is a component of the intraflagellar transport (IFT) complex B (Beales et al., 2007 [PubMed 17468754]). The IFT is essential for the development and maintenance of motile and sensory cilia.[supplied by OMIM]

Database

DATA IMAGES

Anti-IFT80 antibody used in IHC (Paraffin sections) (IHC-P). GTX109393

GTX109393 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using IFT80(GTX109393) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IFT80 antibody used in Western Blot (WB). GTX109393

GTX109393 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
5% SDS PAGE
GTX109393 diluted at 1:500

Anti-IFT80 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109393

GTX109393 ICC/IF Image

IFT80 antibody detects IFT80 protein at centrosome by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: IFT80 protein stained by IFT80 antibody (GTX109393) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169