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IFT88 antibody, C-term

Anti-IFT88 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89425
Anti-IFT88 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89425
Anti-IFT88 antibody, C-term used in Western Blot (WB). GTX89425
Anti-IFT88 antibody, C-term used in Flow cytometry (FACS). GTX89425
Anti-IFT88 antibody, C-term used in Western Blot (WB). GTX89425

Cat. No. GTX89425

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all IFT88 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
ICC/IF 10μg/ml
IHC-P 3μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the golgi/ cytoplasm of HepG2 cells.

IHC-P
Human Kidney shows staining of the brush border in PCT.
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89425-PEP).

Product Note

This antibody is expected to recognise many reported isoforms.

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intraflagellar transport 88 , D13S1056E , DAF19 , TG737 , TTC10 , hTg737

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-IFT88 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89425

GTX89425 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX89425 IFT88 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-IFT88 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89425

GTX89425 IHC-P Image

IHC-P analysis of human kidney using GTX89425 IFT88 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 3μg/ml

Anti-IFT88 antibody, C-term used in Western Blot (WB). GTX89425

GTX89425 WB Image

WB analysis of HepG2 (A) and NIH-3T3 (B) cell lysate using GTX89425 IFT88 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-IFT88 antibody, C-term used in Flow cytometry (FACS). GTX89425

GTX89425 FACS Image

FACS analysis of PFA fixed HepG2 cells using GTX89425 IFT88 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-IFT88 antibody, C-term used in Western Blot (WB). GTX89425

GTX89425 WB Image

WB analysis of human kidney lysate using GTX89425 IFT88 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399