ICC/IF analysis of PFA-fixed HEK293 cells using GTX03348 IGF1 antibody and unimmunized goat IgG (10μg/ml) as isotype control (-ve).
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

FACS analysis of PFA-fixed A549 cells using GTX03348 IGF1 antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

WB analysis of human uterus (Lane A) and negative control HepG2 (Lane B) lysate (35μg protein in RIPA buffer) using GTX03348 IGF1 antibody.
Dilution : 0.5μg/ml

IHC-P analysis of human spleen tissue using GTX03348 IGF1 antibody.
Dilution : 3.75μg/ml
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6.