GeneTex

  • Country / Location Selection

United States (US)

IGF1 antibody

Anti-IGF1 antibody used in Western Blot (WB). GTX100521
Anti-IGF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100521
Anti-IGF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100521
Anti-IGF1 antibody used in Western Blot (WB). GTX100521

Cat. No. GTX100521

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Armadillo
Package
100 μl ($399),
25 μl ($169)
See all IGF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 1 , IGF , IGF-I , IGFI , MGF

Cellular Localization

Secreted

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-IGF1 antibody used in Western Blot (WB). GTX100521

GTX100521 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (GTX100521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100521

GTX100521 ICC/IF Image

IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1 protein stained by IGF1 antibody (GTX100521) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-IGF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100521

GTX100521 IHC-P Image

IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGF1 antibody (GTX100521) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IGF1 antibody used in Western Blot (WB). GTX100521

GTX100521 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (GTX100521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Deng M et al. Cell Res 2022; 32 (7) : 670-686 Lepr(+) mesenchymal cells sense diet to modulate intestinal stem/progenitor cells via Leptin-Igf1 axis.
Hess S et al. Cell Rep Med 2022; 3 (11) : 100820 In?vivo partial reprogramming by bacteria promotes adult liver organ growth without fibrosis and tumorigenesis.
Application : WB
Reactivity : Armadillo
Wang S et al. Oncogene 2019; Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model.
Application : WB, IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for IGF1 antibody (GTX100521). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production