*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
PC-3 , U87-MG , SK-N-SH
Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Insulin Like Growth Factor 1,Igf,Igf-I,Igfi,Mgf,Igf1
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
GTX100521 IHC-P Image
IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGF1 antibody (GTX100521) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX100521 ICC/IF Image
IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1 protein stained by IGF1 antibody (GTX100521) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX100521 WB Image
Sample (30 µg of whole cell lysate)
15% SDS PAGE
GTX100521 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.