GeneTex
United States (US)

IGF1 antibody

GTX100521_40807_20141128_IHC_19011506_941.jpg
GTX100521_40807_IFA_19011506_559.jpg
GTX100521_40807_WB_19011506_369.jpg

Cat No. GTX100521

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Like Growth Factor 1,Igf,Igf-I,Igfi,Mgf,Igf1

Cellular Localization

Secreted

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES
GTX100521_40807_20141128_IHC_19011506_941.jpg

GTX100521 IHC-P Image

IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGF1 antibody (GTX100521) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100521_40807_IFA_19011506_559.jpg

GTX100521 ICC/IF Image

IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1 protein stained by IGF1 antibody (GTX100521) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100521_40807_WB_19011506_369.jpg

GTX100521 WB Image

Sample (30 µg of whole cell lysate)
A: PC-3
B: U87-MG
C: SK-N-SH
15% SDS PAGE
GTX100521 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf