GeneTex
United States (US)

IGF1R antibody [alphaIR3]

Cat No. GTX16890

Host Mouse
Clonality Monoclonal
Clone Name alphaIR3
Isotype IgG1
Application ICC/IF, IP, Neut
Reactivity Human
APPLICATION

Application Note

For IF: Use at a concentration of 1 - 5 μg/ml. For IP: Use at an assay dependent dilution. For Neut: Use at a concentration of 1 μg/ml. Not suitable for use in IHC-P or WB. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

155 kDa. ( Note )

Positive Control

HepG2 cells

Specificity/Sensitivity

Recognizes the human IGF-I Receptor. IGF-I Receptor (Ab-1) immunoprecipitates the a and ? subunits of the IGF-I receptor. IGF-I receptor (GTX16890) blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth.
PROPERTIES

Form

Liquid

Buffer

0.05 M sodium phosphate buffer, 0.2% gelatin, and =0.1% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen used was partially purified receptor from human placenta.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Like Growth Factor 1 Receptor,Cd221,Igfir,Igfr,Jtk13,Igf1R

Cellular Localization

Plasma membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

REFERENCE
Package List Price ($)
$ 319